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KMID : 0379119940220030247
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Korean Journal of Mycology
1994 Volume.22 No. 3 p.247 ~ p.253
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Isolation and Characterization of Aα mating locus from Schizophyllum commune
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Abstract
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This study was carried out to isolate and characterize Aα mating locus controlling fruiting body formation directly in the Basidiomycete Schzophyllum commune growing in the North america. Total numbers of genomic library of S. commune UVM 1-34 was about 2×10⁴ cell. About 90% library was appeared to have about 35 kb inserted genome DNA in cosmid pTC20 vector. 6 clones were proved to have positive signal to probes clomes were appeared to have Aα3 mating activity although they had two different restriction patterns. pSC13 containing 5.7 Kb Psr1-fragment of UVM 1-34 Aα3 allele showed about 50% clamp cell formation indicating mating activity when cotransformation was done together with cosmid pTC20.
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